Recently, Extracellular Matrix (ECM) has become extensively researched due to its outstanding biocompatible properties and low immune response during implantation. ECM refers to the complex bio-polymer, which has proven to be a superior biological material applicable in a variety of fields. In order to be utilized it in a human body, especially in medical industry, an ECM material must have all cellular contents removed and be purified adequately. Although there are many decellularization methods that has been researched and performed, these conventional methods are considered to be protracted. They generally cannot perfectly remove DNA,RNA, or cellular contents, so that it is safe to be implanted in human body. To overcome the indicated drawbacks, this study has conducted a three-day process, which preserves the unique properties of ECM. The three-day process completely removes DNA, RNA, nucleus of the cell, and cellular contents. By employing the concept of enzyme reaction kinetics, the rate of enzymes being reacted with ECM and the loss of ECM weight induced by the enzyme, can be determined. Combining this enzyme reaction kinetics with the process allows highly productive ECM decellularization. Within a decade, the ECM decellularized using this method has great potential to be used in various fields, such as, tissue engineering, medical engineering, food, and cosmetics.