Electrodes have been the gold standard for investigating neuronal signaling due to their high sensitivity and temporal resolution. However, the bulkiness of the electrode limits the spatial resolution and robustness of the tool. In this era, optical methods have become a sensible approach to measure the electrical activities of neurons by using an optical probe that transduces the electrical signal into an optical signal. Electrochromic materials such as Prussian blue, iridium oxide and PEDOT were investigated to determine which had the best optical properties to serve as a sensor for the measurement of extracellular action potentials. These materials are known for their biocompatibility, insolubility in water, and stability. We hypothesized that the electrical potential of excitable membranes will modulate the spectral properties of the material which will be detected through a differential photodiode detector. These materials were electrodeposited onto ITO-coated glass slides and their optical properties were characterized. As a model for electrophysiology measurements, a clone line of modified HEK 293 cells that stably express Nav1.3 and KIR 2.1 and generate spontaneous electrical action potential were used. Herein, we demonstrate the ability to detect the extracellular action potentials of HEK 293 cells and evaluate its potential for imaging network of neuronal cells.