Enzymatic Biofuel cells (EBC) are devices converting chemical energy into electrical energy using enzymes as biocatalysts and glucose/alcohol and oxygen as biofuels. The EBC can offer advantages like low-temperature operation, neutral pH, and specification and can use glucose and oxygen belonging to human body as fuel sources for use as implantable devices. In spite of that, there are still unsolved problems. Slow reaction of the corresponding enzyme molecules is one of the main reasons and the problem can be alleviated by embedment of mediator into the biocatalyst because the mediator promote electron transfer, followed by reaction rate. Regarding the enzyme and mediator immobilization, (i) electrostatic attraction and (ii) chemical bonding can be considered. For the purpose, in anode, glucose oxidase (GOx) enzyme and dye mediator are used as the biocatalyst while in cathode, GOx enzyme and iron containing porphyrin mediator (linker) are used. With the adoption of GOx enzyme and mediators, the catalytic activity of the biocatalysts and EBC performance are improved. With the results, we believe that this study will contribute to establish standard protocols for improvements in performance of EBC system.