One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we elaborated an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at Pt electrodes. Our system was tested under different conditions of pH, time and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the Proteus vulgaris urease activity and the ammonia oxidation reaction. A reaction time and concentration dependence on the ammonia oxidation reaction current density was obtained from the system. Results also showed limited ammonia oxidation under high urea concentrations in ~ 2.5 x 109 cfu/mL Proteus vulgaris in synthetic urine.