Carbon dots have garnered attention for their interesting fluorescent properties and their biocompatibility, which make them ideal for applications in biological imaging and drug delivery. Specifically, carbon dots with emission in the yellow or further red-shifted wavelengths provide a promising platform for biological imaging with minimal interference from blood and tissue autofluorescence. Here we present the synthesis and characterization of multicolored inherently functionalized carbon dots from a variety of carbon sources and at multiple synthesis temperatures. The carbon dots were characterized using transmission electron microscopy (TEM), UV-Vis spectroscopy, fluorescence spectroscopy, fluorescence imaging, Fourier Transform Infrared Spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). We found that functionalized carbon dots could be obtained through a single-step synthesis method, and that small changes in synthesis temperature led to detectable red-shifting or blue-shifting of nanoparticle fluorescence. Additionally, the direction of fluorescence change based on temperature was found to vary between carbon sources. These results suggest the potential for synthesis temperature to be utilized as a simple tool for modulating carbon dot fluorescence.