Chiral nanoarchitectonics to mimic beautiful biological structure available in nature by controlled hierarchical self-assembly of chiral molecules as building block have stimulated great interest. Here, we report a facile approach to prepare a new class of twisted chiral nanostructures based on organic nanofiber using beta-lactoglobulin protein templates. This protein possesses a unique properly of self assemble to form twisted nanofibrilar aggregates upon controlled heating at low temperature. The method involves one pot room temperature co-polymerization of 1,5-dihydroxynapthalene and 1,3,5-trimethyl 1,3,5-triazine in aqueous system containing dispersed protein filaments, which results in π electron rich polymer coated twisted nanofiber. Electron microscopic observation performed by SEM and HRTEM clearly demonstrates morphological transcription of twisted protein fibrils (ca. 20 - 30 nm in diameter) to organic twisted nanofibers. CD and UV-visible spectroscopies indicated that heat-treated protein filaments are constructed on the basis of S-chirally ordered molecular structures in aqueous system, which was transformed in organic nanofibers. The original chiral morphology was perfectly maintained after the co-polymerization. It was believe that the enhancement of chiral ordering of protein fiber by incorporation of polymer. We demonstrated a handedness control over the chiral morphology of molecular self-assembly and stabilization of chiral structure through rapid transcription. Further the obtained twisted organic nanofilaments were subjected to calcination at high temperature. A colloidal dispersion of twisted ultrashort carbon nanofibers was achieved in aqueous solution. The study demonstrated a custom design of chiral carbon nanostructures and making them promising platform for chiral optoelectronics.