Date/Time: 04-05-2018 - Thursday - 05:00 PM - 07:00 PM
Dounia Dems1 Julien Graffion1 Sylvain Le Grill1 Thibaud Coradin1 Nicolas Delsuc2 Carole Aimé1

1, Sorbonne Universités, UPMC Univ Paris 06, Collège de France, UMR CNRS 7574, Paris, , France
2, Département de Chimie, Ecole Normale Superieure, PSL Research University, UPMC Univ Paris 06, CNRS, Paris, , France

The activity of biological components depends both on the nature of the chemical signal and on its density and spatial organization.[1] Engineering materials with functional domains (e.g. anisotropic particles, patchy or multi-compartment particles) is of great interest due to their ability to mimic number of analogues in nature.[2] Here we describe a new method to create bi-functional patchy silica particles (SiNP).
Several alkoxysilane precursors were designed and synthesized bearing (i) a self-assembling group (e.g. anthracene) protecting (ii) a function of interest for bioconjugation (amine) and (iii) an alkoxysilane moiety for the transfer of the assemblies at the surface of the SiNP by sol-gel chemistry. Alkoxysilane self-assemblies were analyzed in different solvents by analytical and imaging techniques (DLS, Spectroscopy, (cryo)TEM). This method enabled us to create size-controlled patches at the surface of the particle. Upon deprotection of the self-assembling groups, amines are revealed for the grafting of bioactive ligands. The density of those ligands was tuned by modifying the size of the patches by influencing the alkoxysilane self-assemblies.
A second molecule of interest can be grafted between the patches, simultaneously or in a two-steps synthesis, in order to create bi-functional particles. In this work, the SiNP were used as a multifunctional platform allowing the co-grafting of biomolecules of interest for axonal out-growth, namely laminin-binding domains that are penta-peptide epitopes (IKVAV)[3] and sulfonate groups (SO3- obtained from the grafting of commercial alkoxysilane) that have been shown to interact with positively charged collagen.[4][5]
The underlying motivation for this multi-component approach is to control the collagen scaffold bioactivity by adjusting the laminin epitope concentration via its chemical confinement, while maintaining the favorable mechanical and cell adhesion properties of collagen.

[1] Banani, S. F., Lee, H. O., Hyman, A. A., Rosen, M. K., Nat Rev Mol Cell Biol. 2017, 18, 287.
[2] Du, J., O’Reilly, R. K., Chem. Soc. Rev, 2011, 40, 2402.
[3] Sur, S., Pashuck, E. T., Guler, M. O., Ito, M., Stupp, S. I., Launey, T. Biomaterials 2012, 33, 545.
[4] Aimé, C., Mosser, G., Pembouong, G., Bouteiller, L., Coradin, T. Nanoscale, 2012, 4, 7127.
[5] Bancelin, S., Decencière, E., Machairas, V., Albert, C., Coradin, T., Schanne-Klein, M.-C., Aimé, C. Soft Matter, 2014, 10, 6651.

Meeting Program

5:00 PM–7:00 PM Apr 5, 2018 (America - Denver)

PCC North, 300 Level, Exhibit Hall C-E