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Akshay Modi1 Surendra Verma1 Jayesh Bellare1

1, IIT Bombay, Mumbai, , India

Bioartificial kidney (BAK) is a novel economic and sustainable biotechnological approach to chronic kidney disease patients. Hollow fiber membranes (HFMs) show the excellent mass-transfer properties, which result in their application firstly for dialysis, and consequently for design and development of bioartificial organs including BAK devices. In this study, the extracellular matrices (ECMs) were coated on TPGS-polyethersulfone hollow fiber mixed matrix membranes (CTP HFMs). The coated HFMs were characterized for surface morphology, surface properties, and strength. To evaluate the suitability of these HFMs for BAK devices, the hemocompatibility tests and kidney cells culture study were performed on the lumen-side and outer surface, respectively, of the developed CTP HFMs. To investigate the toxins clearance performance, as desired for hemodialysis, the separation performance including pure water permeability, solute rejection, and uremic toxins clearance was measured. The results obtained in these studies indicated that the developed CTP HFMs were found to be suitable for human blood-contact devices due to lower hemolysis (0.72 ± 0.1 %) and terminal complement complex SC5b-9 concentration (7.89 ± 1.5 ng/mL), and higher blood coagulation times measured for these HFMs than those measured for the commercial Hemoflow F6. The remarkably high growth, attachment and proliferation of kidney cells on the outer surface of CTP HFMs was observed. The results of glucose consumption and MTT cell proliferation assay supported this observation. With regard to the separation performance, ~ 5-fold higher ultrafiltration coefficient was measured for CTP HFMs than that measured for Hemoflow F6. Almost similar solute rejection profiles were recorded for CTP and Hemoflow F6 HFMs. The superior biocompatibility (including hemocompatibility) and separation performance of CTP HFMs can be attributed to the presence of TPGS as an additive, and ECMs coating in HFMs. In conclusion, the extracellular matrices-coated TP HFMs, developed in this study, are potential membrane materials for BAK devices.

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