Here, we present a nanoparticle platform capable of physiological imaging, triggered by excitation in the NIR windows I and II. This will be achieved with a nanoemulsion that is activated with a continuous wave light source at low power densities (~mW/cm2) for optical imaging, phototherapy, fiducial markers and photoacoustic imaging. The visible light produced from photon upconversion in this hybrid platform will enable wide-field monitoring for longer periods of time than current methods (i.e. days compared to minutes) with the same level of resolution as confocal microscopy. It will decrease phototoxicity and background scattering, with the advantages of multiphoton absorption microscopy at a fraction of the cost by eliminating expensive femtosecond pulsed lasers and laser scanners. The proposed nanoemulsions are composed of clinically approved fluorous phases, perfluorinated molecular emitters, and quantum dots (QDs). These nanoemulsions can be tailored in terms of size, surface functionality, and composition, thus facilitating the development of unique solutions to any imaging problems.