NM06.03.02 : VEGF Modified Fluorescent Nanodiamonds—Synthesis and In Vitro Validation

4:00 PM–4:15 PM Apr 3, 2018

PCC North, 200 Level, Room 227 BC

Nicholas Nunn1 Marco Torelli1 Olga Shenderova1 Joseph Backer3 Daria Filonova2 Alexander Kinev2

1, Adamas Nanotechnologies, Raleigh, North Carolina, United States
3, SibTech, Inc., Brookfield, Connecticut, United States
2, Creative Scientist, Inc., Raleigh, North Carolina, United States

Vascular endothelial growth factor (VEGF) is an overexpressed signal protein observed in cancerous cells and is responsible for the uncontrolled angiogenesis that promotes the growth and metastasis of virtually all forms of cancer. As a result, the overexpression of VEGF can be an important biomarker for indicating the presence of cancerous tissues. Fluorescent nanodiamonds have been functionalized via click chemistry mediated attachment with VEGF-A protein. Subsequent in vitro administration and analysis demonstrate effective conjugation and targeting of the VEGF modified diamond to VEGF cellular receptors (VEGF-R). Thus, an effective demonstration of click chemistry mediated conjugation and targeted in vitro administration of fluorescent nanodiamonds is shown, and the results show continued promise for the use of fluorescent nanodiamonds as a targeted fluorescent probe.