SM04.09.04 : Surface Interactions of DNA and Mononucleotides with Sol-Gel Derived Silica Host

5:00 PM–7:00 PM Apr 5, 2018

PCC North, 300 Level, Exhibit Hall C-E

Caner Durucan1 Derya Kapusuz2 1

1, METU, Ankara, , Turkey
2, Gaziantep University, Gaziantep, , Turkey

Double stranded DNA and dAMP (2′-Deoxyadenosine 5′-monophosphate) were encapsulated in silica by sol-gel route. The microstructure of the biomolecule-hostings gels and the chemical interactions between biomolecules and silica host have been investigated. Ethidium bromide (EtBr) intercalation and leach out tests showed revearled a high hydraulic reactivity for encapsulated DNA and dAMP gels due to presence of more silanol groups than plain silica gel. For both biomolecules, no chemical binding occurred with Si core of the silica network. The chemical association between DNA/dAMP and silica host was through phosphate groups and molecular water attached to silanols, acting as a barrier around biomolecules. The helix morphology was found not to be essential for such interaction. BET analyses showed that interconnected, inkbottle shaped mesoporous silica network with an average pore size of 5.6 nm for DNA and 4.8 nm for dAMP containing bulk gels, respectively.