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SM05.05.20 : Decellularization Process of Adipose Tissue and Its Optimization

5:00 PM–7:00 PM Apr 5, 2018

PCC North, 300 Level, Exhibit Hall C-E

Description
Jae Seok Eo1 Namsoo Kim1

1, The University of Texas at El Paso, El Paso, Texas, United States


Extracellular Matrix (ECM) is a non-cellular scaffold that is composed of complex biopolymers. ECM has become a promising biomaterial to be applied in various fields: tissue reconstruction, drug delivery, and implantation. Although ECM material has been extensively researched for more than 50 years, it has still not been optimized for industrialization. In order to manufacture effective ECM material, decellularization is essential. ECM implantation in vivo without proper decellularization would result in adverse host responses with various antigens. Many researchers have proposed different effective decellularization processes that are mostly too long and still can be improved. In this research, the ECM decellularization process has been optimized into three days by using as source the pig's adipose tissue, and using enzymes and relatively strong chemical detergents such as trypsin, sodium deoxycholate, triton-X 100, peracetic-ethanol solution, and isopropanol. This optimized protocol is very efficient to obtain excellent an ECM product. Through the analysis, it was confirmed that in the resultant ECM material, DNA, RNA, and cellular contents have been completely removed from the adipose tissue, while maintaining the core components of ECM including collagen, elastin, laminin, and fibronectin.

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