Multi-layered structures of AZ31/Ti have been successfully fabricated by single pass hot-rolling. The microstructure and mechanical behavior of the AZ31/Ti multilayers are studied by optical microscopy (OM), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), nanoindentation, tensile, and shear tests. The individual sheets were heated to 450°C for 10 min in an argon atmosphere furnace, and then hot-rolled with a single-pass at thickness reductions 25%, 38%, 50%, and 55%. Heat treatment was applied to the rolled samples at 400 °C for 6h, 12h and 24 h in an argon atmosphere. The results showed that the Ti/AZ31 was well bonded. Loading and unloading curves were obtained at AZ31/Ti interface by a nanoindentation test. X-ray diffraction patterns verified that there was no intermetallic compound formed at the interface of the as-rolled and heat-treated samples. EDS results indicated that a thick bonding layer was formed.The shear strength of the interface bonds increases with the heat-treatment.