Haitao Cui1 Shida Miao1 Timothy Esworthy1 Lijie Grace Zhang1

1, George Washington University, Washington, District of Columbia, United States

As a powerful biofabrication technique, 3D bioprinting has been nowadays used to develop various complex structures, but the printed constructs are always static. 4D bioprinting is a highly innovative additive manufacturing process to fabricate pre-designed, self-assembly structures with the ability to transform from one state to another over the time. However, current 3D/4D bioprinting based additive manufacturing technologies are hindered by the lack of advanced smart biomaterials as “inks”. Therefore, the main objective of our research is to develop novel biologically inspired nano or smart inks and advanced 3D/4D bioprinting techniques to fabricate the next generation of complex tissue constructs (such as vascularized tissue and neural tissue). For this purpose, we designed and synthesized innovative biologically inspired nanomaterials and smart natural materials. Through 3D/4D bioprinting in our lab, a series of biomimetic tissue scaffolds were successfully fabricated. Our results show that these bioprinted nano or smart scaffolds have not only improved mechanical properties but also excellent cytocompatibility properties for enhancing various cell growth and differentiation, thus promising for complex tissue/organ regeneration.