Hae Won Hwang1 2 Gayoung Jung1 Sangwook Wu3 Jeong-Yun Sun2 Myoung-Ryul Ok1

1, Korea Institute of Science and Technology, Seoul, , Korea (the Republic of)
2, Seoul National University, Seoul, , Korea (the Republic of)
3, Pukyong National University, Busan, , Korea (the Republic of)

Recently, the concept of tissue-regenerative all-metallic implant system was introduced [1]. The system consists of two disparate biometals: conventional inert biometal (Ti alloys, SUS, etc.) and newly developed biodegradable metal (Mg, Zn, Fe, etc.). Those metallic systems spontaneously generate hydrogen peroxide which promotes angiogenesis and accelerates overall healing process. However, for the clinical application of this technique to bone fixation, long-term generation of hydrogen peroxide is essential in that the new vessel formation into the hematoma usually occurs in 7 to 14 days after the bone fracture. In addition, unlike the in-vitro study reported [1], in-vivo environment around the hydrogen peroxide releasing implant is composed of cells and extracellular matrix; fibrin, for instance, is the most dominant natural material in the hematoma. Therefore, control of the generation rate of hydrogen peroxide and understanding its diffusion characteristics in the extracellular matrix are essential to optimal design of the tissue-regenerative all-metallic implants. In this research, long-term generation of hydrogen peroxide from all-metallic implant systems are realized by delicate materials selection and engineering, and the diffusion characteristics of hydrogen peroxide in the extracellular matrix were analyzed based on the lab-on-chip technology. Also, considering the diffusion characteristics in the extracellular matrix, the response of human umbilical vein endothelial cells (HUVECs) was observed in the microfluidics chip.