2, RMIT, Melbourne, Victoria, Australia
3, RMIT, Melbourne, Victoria, Australia
The design of tissue engineering materials for both orthopaedic and dental implants is a great challenge in terms of desirable mechanical properties, biocompatibility, and osseointegration. Titanium has been an effective implant material due to its excellent strength to weight ratio, corrosion resistance, toughness, and bio-inert oxide surface. Selective laser melting (SLM) is an additive manufacturing process that fabricates constructs based on CAD Files by scanning powdered materials using the thermal energy supplied by a focused and computer controlled laser beam,. SLM allows the generation of complex 3D parts by a layer-wise material addition technique that selectively melts successive layers of metal powder on top of each other. This work reports the relationship between the cellular attachment and bacterial growth, simulated body fluid (SBF) growth with different inclined SLM part. The SLM printed Ti6Al4V samples were characterized first by the electron microscopy, Profilometer, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy. Cell viability, cell attachment, proliferation of chinese hamster ovarian cells (CHO) on Ti6Al4V SLM plates were confirmed by MTT assay, and confocal imaging in vitro experiments. Bacterial adhesion on SLM parts were also observed by scanning electron microscope and confocal laser microscopy. SLM printed samples were then incubated into SBF solution for 28 days and SBF growth on SLM parts were confirmed by SEM and XPS analysis.