Dominique Ingato1 Julius Edson1 Michael Zakharian1 Young Jik Kwon1

1, University of California, Irvine, Irvine, California, United States

Issues with poor scalability of production have hindered the progression of extracellular vesicle (EV) technology for therapeutic applications. We demonstrate increased cellular production of nano-sized EVs by an order of magnitude compared to naturally occurring EV production by using a method that involves sulfhydryl-blocking. Nanovesicles produced using sulfhydryl-blocking are approximately 50 nm in diameter and can be loaded with therapeutics or engineered via surface modification to achieve desired therapeutic applications. We demonstrate the use of nanovesicles produced by sulfhydryl-blocking for chemotherapeutic delivery of doxorubicin in vivo and for immunotherapy by stimulation of T cells.