Yaniv Olshansky1 2 Robert A Root1 Masaphy Segula3 Giora Rytwo2

1, University of Arizona, Tucson, Arizona, United States
2, MIGAL – Galilee Research Institute, Kiryat Shmona, , Israel
3, MIGAL- Galilee Research Institute, Kiryat Shmona, , Israel

Laccase, a multicopper oxidase, can be used in water treatment processes for the removal of pollutants. This study presents activity of laccase from Rhus vernicifera adsorption on sepiolite and sepiolite modified with chitosan and Cu(II). Adsorption of laccase on raw sepiolite increased its activity by 250±40% compared to the non-adsorbed enzyme, whereas for Cu–S9–chitosan and S9–chitosan activity enhancement was by up to 700% and 500%, respectively. The stronger enhancement for the Cu-containing sorbent suggests that exchangeable Cu has some effect on the adsorbed laccase. Desorption of the adsorbed laccase was less than 10%, and the non-desorbed enzyme retained high activity. This study suggests that chitosan-sepiolite based composites might be used as efficient support for laccase immobilization, resulting in an effective active sorbent for organic pollutants sensitive to laccase oxidative activity.