Sound can be music to please the ear, however, the waves produced can be utilized as “Acoustic Tweezers” for the manipulation of cells and particles in a fluid medium. The acoustofluidics technology, combining sound waves with fluidics, becomes a revolutionary way to dexterously and noninvasively manipulate biological specimens. Firstly, this technique manipulates cells or particles using gentle mechanical vibrations. These vibrations create a pressure gradient in the medium to move suspended micro-objects yielding a contamination-free, contactless, and label-free manipulation. Secondly, acoustofluidics has minimal impact on cell viability and function. Thirdly, this technology can operate in a single micro-device without any external moving parts or complicated setups, which offer additional advantages in ease of use, versatility, and portability. Here, we report a series of acoustic tweezers for the manipulation of micro-objects in a liquid medium or the microfluidic environment to address the problems in the field of biomedicine including tissue engineering, cell-cell interaction, disease diagnostics, and point-of-care testing.